217594.fig.001a
(a)
217594.fig.001b
(b)
217594.fig.001c
(c)
Figure 1: Polyploidization associated with cellular senescence in TIG-1 cells. (a) Differential interference contrast images overlaid with colouring reaction by senescence-associated β-galactosidase. (b) DNA histograms obtained by flow cytometer. (c) Change in frequencies of polyploid cells (with 8C DNA) by population doubling levels in serially passaged cells (left panel) and in cells exposed to 200 μM H2O2 (right panel).