217594.fig.003a
(a)
217594.fig.003b
(b)
217594.fig.003c
(c)
217594.fig.003d
(d)
Figure 3: Localization of p53 protein in mitotic cells. (a) Representative mitotic cells showing localization of centrosomes and p53-positive foci from early passages (upper panels), late passages (middle panels), and the 4th day after exposure to H2O2 (lower panels). Arrows indicate centrosomes with p53 proteins. Chromosomes (blue) and centrosomes (red) are labelled as in Figure 2, and p53 protein is labelled with anti-p53 antibody (green). (b) Mean numbers of p53-positive foci in mitotic cells. Each column is the mean of at least 50 mitotic cells from 4 to 6 experiments. ** with unpaired t-test. (c) Mean frequencies of p53-positive centrosomes. Each column is the frequency calculated using pooled data from 4 to 6 experiments. ** with Chi-square test. (d) Mean frequencies of p53-positive centrosomes according to centrosome numbers per cell. Each column is the frequency calculated using pooled data from 4 to 6 experiments. ** with Chi-square test.