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Figure 4: Localization of p53 phosphorylated at Ser15. Representative mitotic cells showing centrosomal localization of phosphorylated p53 (pSer 15) from early passages (upper panels), late passages (middle panels), and the 4th day after exposure to H2O2 (lower panels). Chromosomes (blue) and centrosomes (red) are labelled as in Figure 2, and phosphorylated p53 (pSer 15) is labelled with antiphosphorylated p53 (pSer 15) antibody (green).