Figure 2: Effects of LDR on renal Akt function in diabetic mice. Renal tissues from mice exposed to sham, single 25 mGy, 75 mGy or 3 × 25 mGy of X-rays were collected at 1, 3, 6, 9, and 12 h after LDR to localize the phosphorylated Akt (p-Akt) at Ser473 with immunohistochemical staining ((a), the representative images of the staining were from the group of 75 mGy of LDR). The expression levels of total Akt and p-Akt were also examined by western blotting (b), followed by quantitative analysis (c). Data was presented as mean ± SD. versus DM. Definition of the abbreviations is provided in Figure 1 legend.