About this Journal Submit a Manuscript Table of Contents
Oxidative Medicine and Cellular Longevity
Volume 2012 (2012), Article ID 310534, 8 pages
http://dx.doi.org/10.1155/2012/310534
Research Article

Persistent Amplification of DNA Damage Signal Involved in Replicative Senescence of Normal Human Diploid Fibroblasts

1Department of Radiation Medical Sciences, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8523, Japan
2Laboratory of Radiation Biology, Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8570, Japan
3Radiation Biology Center, Kyoto University, Kyoto 606-8501, Japan

Received 1 June 2012; Revised 26 July 2012; Accepted 13 August 2012

Academic Editor: William C. Burhans

Copyright © 2012 Masatoshi Suzuki et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. L. Hayflick and P. S. Moorhead, “The serial cultivation of human diploid cell strains,” Experimental Cell Research, vol. 25, no. 3, pp. 585–621, 1961. View at Scopus
  2. E. Sikora, T. Arendt, M. Bennett, and M. Narita, “Impact of cellular senescence signature on ageing research,” Ageing Research Reviews, vol. 10, no. 1, pp. 146–152, 2011. View at Publisher · View at Google Scholar · View at Scopus
  3. S. Misri, S. Pandita, R. Kumar, and T. K. Pandita, “Telomeres, histone code, and DNA damage response,” Cytogenetic and Genome Research, vol. 122, no. 3-4, pp. 297–307, 2009. View at Publisher · View at Google Scholar · View at Scopus
  4. A. Smogorzewska, J. Karlseder, H. Holtgreve-Grez, A. Jauch, and T. De Lange, “DNA ligase IV-dependent NHEJ of deprotected mammalian telomeres in G1 and G2,” Current Biology, vol. 12, no. 19, pp. 1635–1644, 2002. View at Publisher · View at Google Scholar · View at Scopus
  5. J. Karlseder, D. Broccoli, D. Yumin, S. Hardy, and T. De Lange, “p53- and ATM-dependent apoptosis induced by telomeres lacking TRF2,” Science, vol. 283, no. 5406, pp. 1321–1325, 1999. View at Publisher · View at Google Scholar · View at Scopus
  6. V. Gorbunova and A. Seluanov, “Making ends meet in old age: DSB repair and aging,” Mechanisms of Ageing and Development, vol. 126, no. 6-7, pp. 621–628, 2005. View at Publisher · View at Google Scholar · View at Scopus
  7. F. Di Fagagna, P. M. Reaper, L. Clay-Farrace et al., “A DNA damage checkpoint response in telomere-initiated senescence,” Nature, vol. 426, no. 6963, pp. 194–198, 2003. View at Publisher · View at Google Scholar · View at Scopus
  8. O. A. Sedelnikova, I. Horikawa, D. B. Zimonjic, N. C. Popescu, W. M. Bonner, and J. C. Barrett, “Senescing human cells and ageing mice accumulate DNA lesions with unrepairable double-strand breaks,” Nature Cell Biology, vol. 6, no. 2, pp. 168–170, 2004. View at Publisher · View at Google Scholar · View at Scopus
  9. A. Meier, H. Fiegler, P. Mũoz et al., “Spreading of mammalian DNA-damage response factors studied by ChIP-chip at damaged telomeres,” EMBO Journal, vol. 26, no. 11, pp. 2707–2718, 2007. View at Publisher · View at Google Scholar · View at Scopus
  10. M. Suzuki and D. A. Boothman, “Stress-induced premature senescence (SIPS)-influence of sips on radiotherapy,” Journal of Radiation Research, vol. 49, no. 2, pp. 105–112, 2008. View at Publisher · View at Google Scholar · View at Scopus
  11. K. Suzuki, I. Mori, Y. Nakayama, M. Miyakoda, S. Kodama, and M. Watanabe, “Radiation-induced senescence-like growth arrest requires TP53 function but not telomere shortening,” Radiation Research, vol. 155, no. 1, pp. 248–253, 2001. View at Scopus
  12. M. Suzuki, K. Suzuki, S. Kodama, and M. Watanabe, “Interstitial chromatin alteration causes persistent p53 activation involved in the radiation-induced senescence-like growth arrest,” Biochemical and Biophysical Research Communications, vol. 340, no. 1, pp. 145–150, 2006. View at Publisher · View at Google Scholar · View at Scopus
  13. U. Herbig, W. A. Jobling, B. P. C. Chen, D. J. Chen, and J. M. Sedivy, “Telomere shortening triggers senescence of human cells through a pathway involving ATM, p53, and p21CIP1, but not p16INK4a,” Molecular Cell, vol. 14, no. 4, pp. 501–513, 2004. View at Publisher · View at Google Scholar · View at Scopus
  14. G. P. Dimri, X. Lee, G. Basile et al., “A biomarker that identifies senescent human cells in culture and in aging skin in vivo,” Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 20, pp. 9363–9367, 1995. View at Publisher · View at Google Scholar · View at Scopus
  15. M. Yamauchi, Y. Oka, M. Yamamoto et al., “Growth of persistent foci of DNA damage checkpoint factors is essential for amplification of G1 checkpoint signaling,” DNA Repair, vol. 7, no. 3, pp. 405–417, 2008. View at Publisher · View at Google Scholar · View at Scopus
  16. G. Kashino, S. Kodama, Y. Nakayama et al., “Relief of oxidative stress by ascorbic acid delays cellular senescence of normal human and Werner syndrome fibroblast cells,” Free Radical Biology and Medicine, vol. 35, no. 4, pp. 438–443, 2003. View at Publisher · View at Google Scholar · View at Scopus
  17. N. Ohtani, D. J. Mann, and E. Hara, “Cellular senescence: its role in tumor suppression and aging,” Cancer Science, vol. 100, no. 5, pp. 792–797, 2009. View at Publisher · View at Google Scholar · View at Scopus
  18. M. Suzuki, K. Suzuki, S. Kodama, and M. Watanabe, “Phosphorylated histone H2AX foci persist on rejoined mitotic chromosomes in normal human diploid cells exposed to ionizing radiation,” Radiation Research, vol. 165, no. 3, pp. 269–276, 2006. View at Publisher · View at Google Scholar · View at Scopus
  19. E. J. Hall, “Molecular biology in radiation therapy: the potential impact of recombinant technology on clinical practice,” International Journal of Radiation Oncology Biology Physics, vol. 30, no. 5, pp. 1019–1028, 1994. View at Scopus
  20. K. V. A. Thompson and R. Holliday, “Chromosome changes during the in vitro ageing of MRC 5 human fibroblasts,” Experimental Cell Research, vol. 96, no. 1, pp. 1–6, 1975. View at Scopus
  21. P. A. Benn, “Specific chromosome aberrations in senescent fibroblast cell lines derived from human embryos,” American Journal of Human Genetics, vol. 28, no. 5, pp. 465–473, 1976. View at Scopus
  22. A. J. Nakamura, Y. J. Chiang, K. S. Hathcock et al., “Both telomeric and non-telomeric DNA damage are determinants of mammalian cellular senescence,” Epigenetics & Chromatin, vol. 1, p. 6, 2008. View at Publisher · View at Google Scholar
  23. J. P. Murnane, “Telomere dysfunction and chromosome instability,” Mutation Research, vol. 730, no. 1-2, pp. 28–36, 2012. View at Publisher · View at Google Scholar
  24. D. A. Alcorta, Y. Xiong, D. Phelps, G. Hannon, D. Beach, and J. C. Barrett, “Involvement of the cyclin-dependent kinase inhibitor p16 (INK4a) in replicative senescence of normal human fibroblasts,” Proceedings of the National Academy of Sciences of the United States of America, vol. 93, no. 24, pp. 13742–13747, 1996. View at Publisher · View at Google Scholar · View at Scopus
  25. J. N. Sarkaria, R. S. Tibbetts, E. C. Busby, A. P. Kennedy, D. E. Hill, and R. T. Abraham, “Inhibition of phosphoinositide 3-kinase related kinases by the radiosensitizing agent wortmannin,” Cancer Research, vol. 58, no. 19, pp. 4375–4382, 1998. View at Scopus
  26. R. Araki, R. Fukumura, A. Fujimori et al., “Enhanced phosphorylation of p53 serine 18 following DNA damage in DNA- dependent protein kinase catalytic subunit-deficient cells,” Cancer Research, vol. 59, no. 15, pp. 3543–3546, 1999. View at Scopus