Honey Supplementation in Spontaneously Hypertensive Rats Elicits Antihypertensive Effect via Amelioration of Renal Oxidative Stress

Table 3

Gene-specific primers for all the genes investigated.

Gene	Genbank accession no	Primer sequence	PCR product (bp)	Annealing temp. (°C)
CuZnSOD	NM017050	F: 5′-GCAGAAGGCAAGCGGTGAAC-3′	447	62
		R: 5′-TAGCAGGACAGCAGATGAGT-3′
MnSOD	NM017051	F: 5′-CTGAGGAGAGCAGCGGTCGT-3′	258	68
		R: 5′-CTTGGCCAGCGCCTCGTGGT-3′
CAT	BC081853	F: 5′-GCGAATGGAGAGGCAGTGTAC-3′	652	67
		R: 5′-GAGTGACGTTGTCTTCATTAGCACTG-3′
GPx1	M21210	F: 5′-CTCTCCGCGGTGGCACAGT-3′	290	57
		R: 5′-CCACCACCGGGTCGGACATAC-3′
GST-π	L29427	F: 5′-GGAGGAGGTGGTTACCATAGATGTC-3′	389	68
		R: 5′-GCAGGTCCAGCAAGTTGTAATCTGC-3′
GST-α 1/2	AF111160	F: 5′-CCACCTGCTGGAACTTCTCCTCTAT-3′	99	68
		R: 5′-AGGCTGCTGATTCTGCTCTTGAAGG-3′
Nrf2	NM031789	F: 5′-CCATTTACGGAGACCCAC-3′	448	55
		R: 5′-TGAGCGGCAACTTTATTC-3′
β-actin	NM031144	F: 5′-CTGACCGAGCGTGGCTAC-3′	505	58
		R: 5′-CCTGCTTGCTGATCCACA-3′