Protective Role of Malvidin-3-Glucoside on Peroxynitrite-Induced Damage in Endothelial Cells by Counteracting Reactive Species Formation and Apoptotic Mitochondrial Pathway

Figure 6

The activation of caspases-3 and -9 in endothelial cells submitted to peroxynitrite injury is significantly inhibited by the preincubation of cells with 25 𝜇M Mv3glc, for 14 h. BAECs were treated with 500 𝜇M peroxynitrite after this preincubation step, as described in Section 2, and graphs show the kinetic activities of cellular caspase-3 (a) and caspase-9 (b) induced by peroxynitrite treatment (ONOO⁻) in the absence and presence of anthocyanin. Values are mean ± SEM of five different experiments, each one assayed in duplicate. For caspase-3, **𝑃<0.01, ***𝑃<0.001 versus Control; ^#𝑃<0.05 versus ONOO⁻. For caspase-9: ***𝑃<0.001 versus control; ^#𝑃<0.05, ^##𝑃<0.01 versus ONOO⁻.

(a)

(b)