Figure 7: Mv3glc reduced the increase in Bax cytoplasmatic levels induced by peroxynitrite. BAEC were preincubated with 25 M Mv3glc with no further treatment or post-treated with peroxynitrite for 10 min, as described in Section 2 and maintained during 6 h in a serum-free medium, at 37°C. Cytoplasmatic total protein extracts were then analyzed by immunoblot with specific antibody against Bax. Representative blots and densitometric quantifications of three independent experiments are shown. Results were normalized to endogenous -actin and expressed as mean ± SEM of percentage of control. Values are mean ± SEM of four experiments, each one assayed in duplicate. *, **, *** versus Control; # versus ONOO−.