Figure 4: (a) Effects of ISL on intracellular ROS level in B16F0 cells data represent three separate experiments, , and compared with control group (0 μg/mL). Photo of DCF fluorescence in cell was also taken at a 400x magnification parallel to ISL-treatment. (b) Phase-contrast micrographs of B16F0 cells showed the morphology changes and melanin pellet (×400) after treated by 15 μg/mL ISL alone or combined with 200 μM NAC/BSO pretreatment. (c) Effects of 200 μM NAC/BSO pretreatment with or without 15 μg/mL ISL treatment on extracellular melanin levels in B16F0 cells. (d) Effects of 200 μM NAC/BSO pretreatment with or without 15 μg/mL ISL treatment on intracellular melanin levels in B16F0 cells.