608478.fig.003a
(a)
608478.fig.003b
(b)
Figure 3: (a) Intensity normalized ratio image of a Grx1-roGFP2 transgenic worm. Color represents oxidized/reduced Grx1-roGFP2 ratio values, while color saturation represents fluorescence intensities. Spermatheca show clearly reduced GSSG/GSH ratios. (b) Corresponding brightfield image. Construction of transgenic strains, confocal microscopy, and image analysis was performed as described in [133].