The Role of Hydrogen Peroxide in Environmental Adaptation of Oral Microbial Communities

Figure 1

Expression of spxB in freshly isolated human plaque. To detect the expression of spxB among streptococcal species in the oral biofilm, plaque samples were collected from a healthy subject without active caries. Bacterial RNA was isolated and cDNA synthesized after standard protocols [59]. The spxB gene was PCR amplified from the synthesized cDNA with primers described by us earlier specific for spxB and 16S rRNA [59]. Samples were removed during the PCR run after 15, 20, 25, and 30 PCR cycles and loaded on an agarose gel for visualization. 1 = 16S rRNA; 2 = spxB; no RT = control for chromosomal DNA contamination.