DNA Mismatch Repair System: Repercussions in Cellular Homeostasis and Relationship with Aging
Figure 2
Comparisons between MLH1 levels and microsatellite instability. The figure shows the correlations between MSI and MLH1 protein levels, found in the female (F) and male (M) bat lung samples. The correlations were performed considering the relative optical densities (RODs) obtained from the densitometries of the PCR products from DNA microsatellite sequences (MS3EO2 for M. velifer and DESMO1 for D. rotundus), and the MLH1 protein levels were determined by Western Blot analysis in both species. Young bats were given the arbitrary value of one, in order to compare them with the other age groups. Each age group consisted of 5 invidious (n = 5) either male or female bats. One-way ANOVA tests, followed by Tukey-Kramer variance analyses, were used to compare among groups, by sex and age. A 0.05 level of probability was used as a minimum criterion of significance in all analyses. The figures correspond to: (a) Myotis velifer (MS3EO2 versus MLH1) and (b) Desmodus rotundus (DESMO1 versus MLH1).
