750963.fig.002a
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750963.fig.002b
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750963.fig.002c
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750963.fig.002d
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750963.fig.002e
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Figure 2: SA-β-gal staining of 2BS cells grown from PD30 in DMEM supplemented with 1 mg/mL or 2 mg/mL pine pollen (PP). Cells of none-confluent state were washed with PBS, fixed with 3% formaldehyde, and stained in staining solution containing 1 mg/mL 5-bromo-4-chloro-3-indolyl-β-D-galactoside for 16 h. 2BS cells at PD30 were set as young control. Cells were microphotographed at a magnification of 10 × 10. versus 30PD control group; versus 55PD control group.