Antiaging Effect of Pine Pollen in Human Diploid Fibroblasts and in a Mouse Model Induced by D-Galactose

Table 3

Effect of pine pollen on SOD activity and MDA levels in mice.

Group	Treatment	Serum MDA (nmol/mL)	Cerebral MDA (nmol/mg prot)	Serum SOD (U/mL)	Cerebral SOD (Ul/mg prot)
I	Young control	7.74±1.04	1.17±0.45	82.91±4.25	128.98±17.78
II	D-gal (100 mg/kg)	17.66±7.93 **	2.90±1.28 **	69.18±6.00 *	91.36±19.83 *
III	D-gal + PP (500 mg/kg)	9.27±0.89^▵	1.73±0.59^▵	79.06±2.36	109.27±12.91^▵
IV	D-gal + PP (1000 mg/kg)	10.69±2.44^▵	1.64±0.39^▵	77.73±2.65	110.76±24.02
V	D-gal + PP (1500 mg/kg)	9.14±4.07^▵	1.51±0.22^▵▵	78.64±2.44	111.71±16.59^▵
VI	D-gal + AG (100 mg/kg)	5.89±2.65^▵▵	1.48±0.24^▵▵	79.80±2.75^▵	120.88±25.91^▵

Note. C57BL/6J female mice (5-month-old, 𝑛=10 in each group) were treated daily with PBS (s.c, young control), D-galactose (D-gal, 100 mg/kg, s.c), D-gal (100 mg/kg, s.c) with pine pollen (PP, 500, 1000, 1500 mg/kg, ig), and aminoguanidine (AG, 100 mg/kg, ig). SOD and MDA level in sera and brains were determined by commercial kits (Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturer’s protocol. Statistical significant difference: ^∗𝑃<0.05, versus I; ^▵𝑃<0.05, versus II; ^∗∗𝑃<0.01, versus I; ^▵▵𝑃<0.01, versus II.