Table 3: Effect of pine pollen on SOD activity and MDA levels in mice.

GroupTreatmentSerum MDA (nmol/mL)Cerebral MDA (nmol/mg prot)Serum SOD (U/mL)Cerebral SOD (Ul/mg prot)

IYoung control
IID-gal (100 mg/kg) ** ** * *
IIID-gal + PP (500 mg/kg)
IVD-gal + PP (1000 mg/kg)
VD-gal + PP (1500 mg/kg)
VID-gal + AG (100 mg/kg)

Note. C57BL/6J female mice (5-month-old, in each group) were treated daily with PBS (s.c, young control), D-galactose (D-gal, 100 mg/kg, s.c), D-gal (100 mg/kg, s.c) with pine pollen (PP, 500, 1000, 1500 mg/kg, ig), and aminoguanidine (AG, 100 mg/kg, ig). SOD and MDA level in sera and brains were determined by commercial kits (Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturer’s protocol. Statistical significant difference: , versus I; , versus II; , versus I; , versus II.