Preconditioning and Acute Effects of Flavonoids in Protecting Cardiomyocytes from Oxidative Cell Death
Figure 2
Contributions of oxygen, glucose, and serum removal on ischemia-reperfusion-induced cell death in H9c2 cells. Cell viability was assessed by the MTT assay as described in Section 2. The condition of the experiment for different groups was as follows: Control, cells were kept in the control environment (presence of oxygen) and received DMEM plus FBS throughout the experiment. MR/R (medium restriction/reperfusion), cells were placed in the control environment (presence of oxygen) and received ischemia solution during the time of ischemia and DMEM plus FBS during the time of reperfusion. HSR/R (hypoxia-serum restriction/reperfusion), cells were placed in the ischemia environment (absence of oxygen) and received ischemia solution plus glucose (N2-saturated) during hypoxia and transferred to the control environment (presence of oxygen) and treated with DMEM plus FBS during reperfusion. I/R (ischemia/reperfusion), cells were placed in the ischemia environment (absence of oxygen) and received ischemia solution during ischemia and transferred to the control plate (presence of oxygen) where they received DMEM plus FBS during reperfusion. H/R (hypoxia/reperfusion), cells were kept in the ischemia environment (absence of oxygen) during ischemia and transferred to the control plate (presence of oxygen) during reperfusion but received DMEM plus FBS throughout the experiment. Bars represent means of 3–6 wells ± SEM. a–dLetters that are different indicate a significant difference at .
