Preconditioning and Acute Effects of Flavonoids in Protecting Cardiomyocytes from Oxidative Cell Death
Figure 4
Protection by 3-day pretreatment with catechin, epigallocatechin gallate, or proanthocyanidins of H9c2 cells exposed to ischemia-reperfusion. Confluent cells were kept in 2% FBS-DMEM for 6 days including 3-day pretreatment with various concentrations of catechin (results from Figure 3 but with an additional point at 100 μM), epigallocatechin gallate, or proanthocyanidins, and then ischemia-reperfusion groups were exposed to 5 h ischemia followed by 18 h reperfusion. Catechin, epigallocatechin gallate, or proanthocyanidins were present during the reperfusion time. The number of live cells was counted after staining cells with acridine orange. Data were expressed as percent protection against cell death in treatment groups compared to the control nonischemic and ischemia-reperfusion conditions. Each point represents means of 3-4 wells ± SEM. *, †, and ‡ Show versus nontreated ischemic-reperfused condition for catechin, epigallocatechin gallate, and proanthocyanidins, respectively. There were no significant effects of the flavonoids on growth and viability of the cells under the control (non-IR) condition (data not shown).
