Figure 3: Representative Southern blot analysis of control young HDFs, SIPS, and control senescent HDFs. Telomeric DNA was shown as wide smears in all lanes. The telomere length was represented by mean terminal restriction fragment (TRF) value. Lane 1; molecular weight marker, lane 2; control young HDFs, lane 3; SIPS, lane 4; senescent HDFs, lane 5; molecular weight marker (a). Terminal restriction fragment, (TRF length) of young HDFs, SIPS, and control senescent HDFs. Shortening of telomere length was observed in SIPS and control senescent HDFs (b). adenotes compared to young HDFs, bP<0.05 compared to SIPS. Data is presented as mean SD, .