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Oxidative Medicine and Cellular Longevity
Volume 2012 (2012), Article ID 805850, 7 pages
http://dx.doi.org/10.1155/2012/805850
Clinical Study

Changes in Athlete’s Redox State Induced by Habitual and Unaccustomed Exercise

1Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia
2Department of Physiology, Faculty of Sport and Physical Education, University of Nis, Carnojeviceva 10A, 18000 Nis, Serbia
3Institute of Physiology “Richard Burian”, School of Medicine, University of Belgrade, Visegradska 26, 11000 Belgrade, Serbia

Received 28 August 2012; Revised 4 November 2012; Accepted 12 November 2012

Academic Editor: Antonio Ayala

Copyright © 2012 Dusica Z. Djordjevic et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purpose of this study was to assess the influence of sport-specific and nonspecific bouts of exercise on athletes’ redox state. Blood samples were collected from 14 handball players immediately before and after graded exercise test on the cycle ergometer and handball training. Levels of superoxide anion radical ( ), hydrogen peroxide (H2O2), nitrites ( ) as markers of nitric oxide, index of lipid peroxidation (TBARs), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) activity were determined. Exercise intensity was assessed by a system for heart rate (HR) monitoring. Average athletes’ HR was not significantly different between protocols, but protocols differed in total time and time and percentage of time that athletes spent in every HR zone. The laboratory exercise test induced a significant increase of H2O2 and TBARs as well as the decrease of the SOD and CAT activity, while after specific handball training, levels of were increased and SOD activity decreased. It seems that unaccustomed short intensive physical activity may induce oxidative stress in trained athletes, while sport-specific activity of longer duration and proper warm-up period may not. Further research should show whether the change of protocol testing and the implementation of various supplementations and manual methods can affect the redox equilibrium.