Overexpression of Fatty-Acid--Oxidation-Related Genes Extends the Lifespan of Drosophila melanogaster
Figure 1
Overexpression of fatty-acid -oxidation-related genes using EP lines. (a) Schematic representation of fatty-acid -oxidation. CG6783 encodes the fatty-acid-binding protein, which mediates the transportation of acyl-CoA to cellular organelles. CG13890 encodes dodecenoyl-CoA delta-isomerase, which modifies cis-enoyl-CoA to trans-enoyl-CoA, a common substrate for enoyl-CoA hydratase in the -oxidation cycle of saturated fatty acyl-CoA esters. (b) Overexpression of CG6783 or CG13890 using EP lines (GX62610, GX4385) and da-Gal4. The mRNA of CG6783 or CG13890 was analyzed in the whole body RNA extract from (black bars) and da/+ (gray bars) flies. Significance was determined via a t-test (**P < 0.001, ***P < 0.0001).
