Overexpression of Fatty-Acid-𝜷-Oxidation-Related Genes Extends the Lifespan of Drosophila melanogaster

Figure 2

Overexpression of CG6783 or CG13890 throughout the whole body extends lifespan, which is associated with diet restriction. (a) Flies exhibiting overexpression of EP ^CG6783 (closed-square line) and EP ^CG13890 (closed-triangule line) fed 16% SY media display increased median and/or maximum lifespan when compared to the da-Gal4 driver alone (open-circular line). Significance was determined via a log-rank test (CG6783, P < 0.001 to da/+, P = 0.6 to EP ^CG6783/+; CG13890, P < 0.1 to da/+, P < 0.05 to EP ^CG13890/+). (b) Flies overexpressing CG6783 using UAS-CG6783 and Act-GS-Gal4 showed increased median lifespan in response to feeding with RU486-containing food from day 3 of adulthood (RU+, closed-symbol line) when compared to the uninduced control (RU−, open-symbol line). Significance was determined via a log-rank test (P < 0.0001). (c) Mortality curves of the flies that overexpressed CG6783 or CG13890. The natural log of the mortality rate was plotted using the Gompertz mortality model. Red lines indicate linear regressions for each category. (d) Overexpression of CG6783 or CG13890 throughout the whole body reduced the lifespan extension with dietary restriction. Survival curves of the flies that overexpressed CG6783 (square line) or CG13890 (triangular line) fed 2% or 16% SY media. (e) Dietary restriction in adult Drosophila when CG6783 or CG13890 are overexpressed throughout the whole body. The median lifespan was calculated from Kaplan-Meier survival analysis of the EP lines fed a range of yeast concentrations. Red dashed lines indicate linear regressions for each line.