Association of Base Excision Repair Gene Polymorphisms with ESRD Risk in a Chinese Population
Figure 1
Demonstration of genotyping and sequence. (a) HRM directly discriminated the heterozygotes (OGG1 c.977 CG) and homozygotes (OGG1 c.977 CC or GG). (b) homozygous PCR products (CC or GG) were measured by LightScanner after being mixed with an equal amount of a known product (CC), which distinguished the wild homozygous samples (CC) from the variant ones (GG), as the mutational homozygotes (GG) were converted into heterozygotes (CG). (c) random samples from OGG1 c.977C > G testing were sequenced for confirmation. (d) The PCR products were separated using 1% agarose gels to assess the pattern of AluYb8 insertion into the MUTYH gene. Lanes 2, 4, 5, and 10: absence/absence (A/A); 3, 6, and 9: absence/presence (A/P); 1, 7, and 8: presence/presence (P/P); M: DNA Marker 2000.
