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Figure 2: Acute ethanol gavage decreases protein nitrosylation after hemorrhage and resuscitation. Immunohistochemical staining for 3-Nitrotyrosine (3-NT) reveals areas of protein nitrosylation. 3-NT-stained, representative liver sections from sham-operated rats are given in (a) (saline control, ctrl) and (e) (acute ethanol gavage, EtOH). 3-NT-stained, representative liver sections from rats at 2 h, 24 h, and 72 h after H/R are given in (c)-(d) (saline control treatment, ctrl) and (f)–(h) (acute ethanol gavage, EtOH); bar is 50 μm.