Rapidly Developing Yeast Microcolonies Differentiate in a Similar Way to Aging Giant Colonies
Figure 2
Localization of cells containing storage compounds (lipid droplets) and cells with active autophagy and TORC1 signaling pathway. (a) Vertical transversal cross-sections of 4-day-old BY4742 microcolonies. Left, boundary between Um and Lm cells, lipid droplets are stained with Nile red. Right, lipid droplets of Um and Lm cells stained with Nile red (red) and cell walls with concanavalin A conjugated with Alexa Fluor 488 (green). (b) Vertical cross-sections of 4- and 7-day-old microcolonies of strains producing cytosolic proteins Ino1p-GFP or Met17p-GFP. Um cells are shown; arrows indicate GFP in vacuoles of 7-day-old Um cells where cytosolic proteins were delivered to vacuoles via autophagy. (c) Vertical cross-sections of 4-day-old microcolonies formed by Gat1p-GFP strain showing localization of Gat1p-GFP protein in Um and Lm cells. Arrows indicate relocalization of Gat1p-GFP from the cytosol to the nuclei of Um cells after treating the cut edge of the colony section with 250 ng/mL rapamycin, an inhibitor of TORC1.
