Novel Hematopoietic Target Genes in the NRF2-Mediated Transcriptional Pathway

Figure 1

NRF2, NQO1, and NFE2 gene expression in K562 and lymphoid GM121878 cells. (a) NRF2 mRNA from K562 and GM12878 cells treated for 24 hours with 10 μM SFN increased compared to control. (b) Known NRF2-regulated gene NQO1 expression significantly increased in both K562 and GM12878 SFN-treated cells. (c) NFE2-specific gene expression indicated that NFE2 was specific to erythroleukemic K562 cells but not inducible upon SFN treatment. Values were normalized to 18S, and fold change (FC) over nontreated (NT) cells was calculated for (b) and (c). Bars display the average of 3 independent experiments ± SEM; ^∗𝑃<0.05, ^∗∗𝑃<0.01.

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