Novel Hematopoietic Target Genes in the NRF2-Mediated Transcriptional Pathway

Figure 3

Stable silencing of KEAP1 (KEAP1KD) in K562 cells. (a) KEAP1KD cells showed an 82.7% decrease in KEAP1 gene expression compared to scrambled control. (b) Basal levels of NRF2-mediated target NQO1 increased 3.2-fold in KEAP1KD cells. Values were normalized to 18S and bars represent an average of 3 independent experiments ± SEM. (c) Total protein from KEAP1KD C1 and C2 cells showed an increase in NQO1 compared to control. A NQO1 band at 31 kDA was confirmed by protein ladders, and the membrane was reprobed with β-actin as a loading control.