Novel Hematopoietic Target Genes in the NRF2-Mediated Transcriptional Pathway

Figure 8

TBXAS1 gene expression in airway, hepatic, erythroid, and lymphoid cell lines. (a) NRF2 silencing in three cell lines (A549, BEAS-2B, and HepG2) treated for 8 hours with 10 μM SFN. TBXAS1 expression was reduced most significantly in A549 followed by HepG2 cells. (b) Chemical activation (10 μM SFN for 24 hours) of NRF2 in K562 and GM12878 cells led to robust induction of TBXAS1. (c) Genetic activation of NRF2 by KEAP1 silencing led to a potent TBXAS1 activation in erythroid K562 cells. Values were normalized to BACT or 18S. Bars represent an average of 3–6 independent experiments ± SEM; ^∗𝑃<0.05, ^∗∗𝑃<0.01.