A Novel Sit4 Phosphatase Complex Is Involved in the Response to Ceramide Stress in Yeast
Figure 2
Deletion of SIT4 or KTI12 confers resistance to excess dihydroceramide. (a) Ceramide growth tests in Sit4 associated protein (SAP) mutants. (b) Ceramide growth tests in Elongator-associated mutants. Yeast cultures were diluted to 5 × 103 cells/mL in YPD with the addition of either 15 μM C-2 dihydroceramide or an equal volume of ethanol. Cells were grown until the untreated culture reached exponential phase and the OD600 of all cultures was determined. Growth in 15 μM dihydroceramide is expressed as a percentage of untreated growth. Raw OD600 values are given in Supplementary Table 1. A minimum of three replicates are shown and error bars represent the standard deviation above and below the mean. A one-way ANOVA with a Bonferroni post-test was used to determine if mutants showed a significant difference in growth compared to the wild type (CY4029) (, , and ).
