A Novel Sit4 Phosphatase Complex Is Involved in the Response to Ceramide Stress in Yeast

Figure 6

Immunoprecipitation of HA-Sit4p reveals novel interactors. Equal amounts of protein extracts were immunoprecipitated with magnetic beads coated with anti-HA antibodies, and the precipitates were then subjected to Western blotting and probed with anti-HA and anti-Tpd3 (a) or anti-c-myc (b) antibodies. Equal amounts of total protein extracts (without immunoprecipitation) were also probed with anti-Tpd3 or anti-c-myc antibodies.