A Novel Sit4 Phosphatase Complex Is Involved in the Response to Ceramide Stress in Yeast

Table 1

Yeast strains used in this study.

Strain	Genotype	Reference
CY4029	Mat a ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 SSD1-v1 gal+	[20]
CY3938	CY4029, sit4Δ::HIS3	[20]
CY5236	CY4029, sap4Δ::LEU2  sap155Δ::HIS3 sap185Δ::ADE2  sap190Δ::TRP1	[20]
CY5220	CY4029, sap4Δ::LEU2 sap155Δ::HIS3	[20]
CY5224	CY4029, sap185Δ::ADE2 sap190Δ::TRP1	[20]
CY4917	CY4029, sap185Δ::ADE2	[20]
CY4380	CY4029, sap190Δ::TRP1	[20]
DJY101	CY4029, sit4Δ::HIS3 kti12ΔKlLEU2	[18]
LFY3	Mat a ade2-1 his3-11,15 leu2-3,112, ura3-1 can1-100, elp1Δ::TRP1	[18]
LFY4	Mat a ade2-1 his3-11,15 leu2-3,112, ura3-1 can1-100, elp2Δ::TRP1	[18]
LFY5	Mat a ade2-1 his3-11,15 leu2-3,112, ura3-1 can1-100, elp3Δ::TRP1	[22]
LFY6	Mat a ade2-1 his3-11,15 leu2-3,112, ura3-1 can1-100, kti12Δ::TRP1	[22]
AWY1	CY4029 TRP1::GAL1::(HA) 3-SIT4 CDC55-(c-myc)3::HIS3MX6	This study
AWY2	CY4029, kanMX6::PGAL1::(HA) 3-PPH21, CDC55-(c-myc) 3::HIS3MX6	This study
AWY3	CY4029, CDC55-(c-myc) 3::HIS3MX6	This study