The Natural Stilbenoid Piceatannol Decreases Activity and Accelerates Apoptosis of Human Neutrophils: Involvement of Protein Kinase C
Figure 4
Phosphorylation of PKCα, PKCβII, and PKCδ in PMA-stimulated neutrophils treated with 10 and 100 μmol/L piceatannol (PIC). Phosphorylated PKC isoforms were isolated by Western blotting and detected with anti-phospho-PKCα/βII (Thr638/641) and anti-phospho-PKCδ (Thr505) antibodies. The values are presented as percentage of resting control. Control values, given as optical density of PKC bands corrected to β-actin content, were (PKCα, βII) and (PKCδ). The representative blot manifests elevated phosphorylation of PKC isoforms in neutrophils stimulated with PMA as well as the effect of piceatannol on this increase. Mean ± SEM, .
