Blocking Type I Interferon Signaling Rescues Lymphocytes from Oxidative Stress, Exhaustion, and Apoptosis in a Streptozotocin-Induced Mouse Model of Type I Diabetes
Figure 1
STZ-induced immunosuppressed diabetic mice. (a) Pancreatic sections of both control and STZ-treated mice were stained with H&E for light microscopy examination (magnification, ×40). (b) Pancreatic sections were immunohistochemically stained for the islet β cells (magnification, ×40). (c) Pancreatic islet β cells were isolated from control and diabetic mice, and the percentage of β cells that underwent apoptosis was monitored using Annexin V binding assay followed by flow cytometry analysis. (d) Control and diabetic mice were immunized with SRBC as described in the text. The level of IgG anti-SRBC level was compared between control and diabetic mice. Histograms show one representative experiment of three. Number in histograms represents the mean fluorescent intensity (MFI).
