Blocking Type I Interferon Signaling Rescues Lymphocytes from Oxidative Stress, Exhaustion, and Apoptosis in a Streptozotocin-Induced Mouse Model of Type I Diabetes
Figure 5
Increased PD-1 expression and lymphocyte exhaustion during diabetes. PBMCs were isolated from 5 mice () per group and stained for 30 minutes at 4°C with PE-conjugated anti-PD-1 or isotype control IgG mAbs. The cells were then washed twice and fixed in PBS containing 2% paraformaldehyde, followed by flow cytometry analysis of PD-1 expression on viable cells. The numbers shown correspond to the mean fluorescence intensity (MFI) of PD-1 expression on labeled cells.
