Ammonium-Dependent Shortening of CLS in Yeast Cells Starved for Essential Amino Acids Is Determined by the Specific Amino Acid Deprived, through Different Signaling Pathways

Figure 2

Tor1p regulates ammonium CLS shortening in response to amino acid starvation. Survival of ((a) and (b)) tor1Δ, ((c) and (d)) ras2Δ, and ((e) and (f)) sch9Δ cells, nonstarved or starved for leucine, histidine, or lysine, individually or all at the same time, upon transfer to water (open symbol) or water with (NH₄)₂SO₄, 0.5% (dark symbols). In all the cultures, starting cell density was about 3.8 × 10⁷ cells/mL, and the initial pH was adjusted to 7.0. Values are means ± SEM (𝑛=3). All time points have error bars; however, for time points with reduced standard error, they are not visible.

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