Ammonium-Dependent Shortening of CLS in Yeast Cells Starved for Essential Amino Acids Is Determined by the Specific Amino Acid Deprived, through Different Signaling Pathways
Figure 4
Ammonium induces Sch9p-dependent Hog1p phosphorylation in starvation conditions. Westernblot analysis of Pi-Hog1p levels present in S. cerevisiae (BY4742) wild-type (WT) and sch9Δ cells starved for (a) all three amino acids or leucine and (b) starved for histidine or lysine, before (T0) and after 20 minutes upon transfer to water (H2O) or water with (NH4)2SO4, 0.5% (). In all the cultures, starting cell density was about 3.8 × 107 and the initial pH was adjusted to 7.0. Control cells were grown on YPD medium (Control-C) and incubated for 5 minutes in YPD medium supplemented with 1 M NaCl.
