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Figure 12: Confocal immunofluorescence imaging of mitochondrial remodeling in MSCs challenged with LPS. Mitochondrial networks were visualized using projections of TOM20 (red channel), a mitochondrial marker. ((a)-(b)) Control MSCs: mitochondrial network is presented by small-size dots. ((c)-(d)) MSCs challenged with LPS: formation of long-length mitochondrial network occurred due to mitochondrial fusion. Conditions: MSCs were incubated with 500 ng/mL LPS for 3 h. The cells were analyzed 24 h after challenge with LPS. Counterstaining of nuclei was with Hoechst 33342 (blue channel). The confocal images were taken with pinhole setup to obtain 0.5 μm Z-sections.