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Figure 3: Confocal immunofluorescence imaging of iNOS protein in MSCs challenged with LPS. (a1) Projections of nuclei (blue channel) in control MSCs. (a2) Projections of iNOS (red channel), (a3) overlay of projections presented in (a1) and (a2) and a respective DIC image. (b1) Projections of nuclei (blue channel) in LPS-challenged MSCs. (b2) Projections of iNOS (red channel) and (b3) overlay of projections presented in (b1) and (b2) and a respective DIC image. A massive accumulation of iNOS occurred in the LPS-challenged MSCs. Counterstaining of nuclei was with Hoechst 33342 (blue channel). The confocal images were taken with pinhole setup to obtain 0.5 μm Z-sections. Conditions were the same as indicated in Figure 2.