Figure 9: Western immunoblot analysis of redox-response and autophagy-mediated proteins in MSCs challenged with LPS. (a) Representative immunoblotting bands of HSP70, Nrf2, p62/SQSTM1, Sirt3, and LC3 proteins. The protein extracts were obtained from MSC cultures 24 h after challenge with LPS. ((b)-(c)) Representative immunoblotting bands of Hemeoxygenase 1 (HO1) protein (b) and respective densitometry histograms of HO1 bands (c) in MSCs stimulated with LPS. The presented bars indicate the relative density of HO1 protein (normalized to density of actin bands). The statistical significance was determined by Student’s -test . Conditions: MSCs were incubated with 500 ng/mL LPS for 3 h. The cells were harvested at 24 h following challenge with LPS.