Senescence-Related Changes in Gene Expression of Peripheral Blood Mononuclear Cells from Octo/Nonagenarians Compared to Their Offspring

Table 5

Genes differentially expressed in PBMC of octo/nonagenarian versus offspring. Statistical analysis was carried out using independent t-test (RT-PCR) with a Benjamini-Hochberg false-discovery rate (microarray).

Biological process	Gene symbol	Gene name	RT-PCR		Microarray data
			Fold change	𝑃 value^a	Fold change	Corrected 𝑃 value
Cell cycle	CDKN1B	Cyclin-dependent kinase inhibitor 1B (p27, Kip1)	1.48	9.46𝐸−03	1.43	4.90𝐸−03
Double-strand break repair	XRCC6	X-ray repair cross-complementing protein 6 (Ku autoantigen, 70 kDa)	1.49	1.34𝐸−02	1.27	2.30𝐸−02
Cell cycle arrest	FOXO4	Forkhead box O4	1.45	3.49𝐸−03	1.27	3.00𝐸−02
Regulation of apoptosis	BAK1	BCL2-antagonist/killer 1	1.91	3.80𝐸−02	1.52	3.90𝐸−04
Response to stress	HSPA8	Heat shock 70 kDa protein 8	−4.01	1.30𝐸−04	−1.47	5.60𝐸−03
Immune response	CD40LG	CD40 ligand	−2.71	1.32𝐸−04	−1.46	1.10𝐸−02
Cell division	CDC14A	CDC14 cell division cycle 14 homolog A	−2.04	4.21𝐸−04	−1.53	4.40𝐸−03
Inflammatory response	TNFSF4	Tumor necrosis factor (ligand) superfamily, member 4	−2.57	4.02𝐸−05	−1.93	4.90𝐸−02
Chromatin modification	NCOR1	Nuclear receptor corepressor 1	−1.69	7.86𝐸−03	−1.43	4.40𝐸−02
Insulin action	PRKCA	Protein kinase C, alpha	−1.64	3.07𝐸−02	−1.38	2.70𝐸−02
Induction of apoptosis	CASP2	Caspase 2	−2.08	3.66𝐸−03	−1.33	3.10𝐸−02
Metabolic process	ATP2A2	ATPase, Ca^++ transporting	−1.61	5.49𝐸−03	−1.37	1.30𝐸−02
Housekeeping gene (cytoskeleton)	ACTB	Beta Actin

aThe 𝑃 values for the real-time reverse transcription PCR (RT-PCR) refer to a two-tailed 𝑡-test for the differences in means between the normalized Ct values in octo/nonagenarians versus offspring.