Carbon Monoxide Attenuates Dextran Sulfate Sodium-Induced Colitis via Inhibition of GSK-3β Signaling
Figure 2
CO attenuates experimental colitis as measured by histology in colon and inflammatory cytokines in colon, MLNs, and BMMs. Mice were administrated with 3% DSS with drinking water for 6 days and CO (250 ppm) inhalation for 4 h daily and injection of LiCl (200 mg/kg, i.p) was performed daily for more 4 days. (a) Colon sections were subjected to H&E staining. (b) TNF-α, iNOS, and IL-10 mRNA levels were detected from colon tissue by RT-PCR. (c) iNOS and pGSK-3β protein levels were measured from colon tissue by western blotting. To detect the levels of cytokines in MLNs and BMM cells, mice were treated with 3% DSS solution for 6 days and isolated MLN and BMM cells were treated with CORM2 (100 μM) or LiCl (20 mM) for 6 h. The levels of TNF-α, iNOS, and IL-10 mRNA were performed by using RT-PCR in MLN cells (d) and BMMs (e) and the levels of pGSK-3β were detected with western blot in MLN (f) and BMM (g) cells. Also, mRNA levels of GATA-3 (h) and t-bet (i) were performed by using real-time RT-PCR in MLN cells. Data represents mean ± SEM, *, **, and ***.
