Comparative Effects of Bone Marrow Mesenchymal Stem Cells on Lipopolysaccharide-Induced Microglial Activation

Figure 2

Identification of nonstimulated and LPS-stimulated microglia. (a) OX42-IR microglia counterstained with Hoechst 33258; (b) >98% cells were OX42-IR microglia analyzed by flow cytometry; (c) and (e) nonstimulated cultures and (d) and (f) LPS-stimulated cultures; (f) denotes iNOS-IR cells; (g) nitrite release to medium in LPS- or combined LPS and IFNγ-treated microglial cultures. LPS doses were ranging from 100 ng/mL to 1 µg/mL. Aliquot of media was collected at 4, 24, or 48 hrs after treatment. Data were means ± SEM, ^∗𝑃<0.05 compared with 0 ng/mL LPS (control) group. ^#𝑃<0.05 compared with 4 hrs treatment. ^‡𝑃<0.05 compared with 24 hrs treatment.

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