Comparative Effects of Bone Marrow Mesenchymal Stem Cells on Lipopolysaccharide-Induced Microglial Activation

<table>Identification of soluble factors released to conditioned media by microglia, BMMSC, and cocultures. (a) Expressions of 29 rat cytokines in the conditioned media. Spots of TIMP-1 (red square) and VEGF (green square) were found to change among treatments. (b) Histogram showing the fold changes of TIMP-1 and VEGF among treatments. (c) Western blot analysis of TIMP-1 and VEGF released in the 24 hrs conditioned medium of all cultures. The Ponceau-S staining (red) of IgG was used as an internal control. (d) Histogram showing the amounts, in <i>μ</i>g/mL, of the TIMP-1 as quantified by ELISA. Determinations were means ± SEM from coculture media subjected to ELISA experiments. <svg height="13.95" id="M13" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 66.324997 13.95" width="66.324997" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>, compared to M group.</table>

Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: Comparative Effects of Bone Marrow Mesenchymal Stem Cells on Lipopolysaccharide-Induced Microglial Activation 