EPR spin-trapping of superoxide with DMPO in hypoxic-reoxygenated bovine aortic endothelial cells. First integral of DMPO-OH, amplitude of the 2nd spectral peak. DMPO-OH is the stable EPR-active adduct of trapped superoxide. All drugs were applied prior to hypoxia. Sivelestat (190 μM) decreased the formation of superoxide (DMPO-OH) in bovine aortic endothelial cells as indicated by spin-trapping with DMPO. Oxypurinol (500 μM) was more effective than sivelestat, and S.O.D. (5 kU/mL) was most effective at decreasing DMPO-OH ( per group).
