Carbon Monoxide Protects against Hepatic Ischemia/Reperfusion Injury via ROS-Dependent Akt Signaling and Inhibition of Glycogen Synthase Kinase 3β
Figure 3
PI3 K blockade restores liver I/R injury in mice pretreated with CO inhalation. Mice were treated with CO gas (CO), LY294002 (LY), or both or vehicle at 30 minutes prior to the liver ischemia insult, as described in Materials and Methods. Liver samples were harvested at 6 hours after-reperfusion. (a) Proteins were analyzed by Western blotting with Abs against phosphorylated or total GSK3β and β-actin. Sixty minutes ischemia time was used to show the effect of PI3 K inhibition in liver I/R injury. , . (b) Average sALT levels in different experimental groups were measured. sALT levels were measured at 6 hours of reperfusion. (c) Representative liver histology (H&E staining) is shown. To establish the functional relationship between PI3 K and GSK3β, CO inhalation was administered 12 hr and LY 30 minutes prior to the ischemic insult and 6 hr after reperfusion. (d) Liver neutrophil accumulation, assessed by MPO activity. Data represent mean ± S.D. ( = 4–6 samples/group). , .
