Attenuation of -Amyloid-Induced Oxidative Cell Death by Sulforaphane via Activation of NF-E2-Related Factor 2
Figure 6
Effect of Nrf2 gene knock-down on SUL-mediated protection against A-induced apoptotic cell death. SH-SY5Y cells were transiently transfected with siRNA of Nrf2 according to the protocol provided by manufacturer and then exposed to A (15 M) in the presence or absence of SUL (5 M) for 24 h. (a) MTT assay was performed to measure cell viability. Data are represented as mean ± S.D. (). , significantly different between groups. (b) TUNEL staining was conducted to verify DNA fragmentation in situ. (A) Vehicle-treated control; (B) A alone (15 M); (C) A (15 M) + SUL (5 M); (D) A (15 M) + SUL (5 M) + Nrf2-siRNA. (c) TMRE staining was performed to compare MMP. Data are represented as mean ± S.D. (). , significantly different between groups. (d) Protein expression of Bcl-2 and Bax was determined by western blot analysis using specific antibodies. Actin levels were examined to ensure equal amount of protein loading.
