The Cell Wall Sensors Mtl1, Wsc1, and Mid2 Are Required for Stress-Induced Nuclear to Cytoplasmic Translocation of Cyclin C and Programmed Cell Death in Yeast

Figure 1

Negative synthetic growth defect in a cnc1Δ gfa1-1 mutant. (a) Model of the cell wall signaling pathway that regulates cyclin C cytoplasmic translocation and consequent degradation. Adapted from [5] and data presented in [6, 7, 25] and this report. (b) An isolate following EMS mutagenesis harboring either a URA3 (left half) or TRP1 (right half) marked CNC1 expression plasmid was streaked on medium containing 5-FOA. (c) and (d) The gfa1-1 cnc1Δ double mutant strain was transformed with the indicated plasmids then streaked on 5-FOA medium. All plates were incubated for 3 days at 30° before the image was taken. (e) Sequence analysis of the gfa1-1 allele is shown. The six nucleotide deletion is indicated by the hash marks. The predicted amino acid sequences for the wild type and gfa1-1 encoded proteins are indicated in single amino acid code.