The Cell Wall Sensors Mtl1, Wsc1, and Mid2 Are Required for Stress-Induced Nuclear to Cytoplasmic Translocation of Cyclin C and Programmed Cell Death in Yeast
Figure 4
Mtl1 is glycosylated on Asn42. Immunoprecipitates from extracts prepared from a wild-type strain expressing Mtl1-3HA from its normal promoter were either subjected (+) or not (−) to Endo H treatment. Molecular weight standards (kDa) are given on the left. The arrowhead indicates the migration of the unmodified Mtl1-3HA. Open and closed arrows indicate modified Mtl1-3HA before and after Endo H treatment, respectively. (b) Site directed mutagenesis was used to substitute alanine for asparagine at position 42 or 547. The experiments described in (a) were repeated with the modified forms of Mtl1. (c) The experiment described in (a) was repeated with a wild type and gfa1-1 mutant. Open and dark grey arrows indicated fully modified form in wild type and gfa1-1 mutant, respectively. Closed and light grey arrows indicate the Endo H treated samples in wild type and the gfa1-1 mutant, respectively.
