The Cell Wall Sensors Mtl1, Wsc1, and Mid2 Are Required for Stress-Induced Nuclear to Cytoplasmic Translocation of Cyclin C and Programmed Cell Death in Yeast
Figure 6
Membrane sensors are not required for cyclin C destruction following 0.8 mM H2O2 stress. (a) Wild type (RSY10), mtl1Δ (RSY1660), mids2Δ wsc1Δ (RSY1547), and mid2Δ wsc1Δ mtl1Δ (RSY1707) strains expressing myc-cyclin C (pLR337) were grown to midlog phase (0 hr) and then treated with 0.8 mM or 1.2 mM H2O2 for the indicated times. Cyclin C levels were determined by Western blot analysis of immunoprecipitates. Tub1 levels were used as a loading control. (b) Phosphorylation (top panels) and immunoprecipitation (bottom panels) of Slt2-HA in wild type, mtl1Δ, mids2Δ wsc1 , and mid2Δ wsc1Δ mtl1Δ strains following 0.8 mM H2O2 treatment. (c) Kinetics of cyclin C degradation are dependent upon the H2O2 dose. Wild type (RSY10) and mtl1Δ (RSY1600) strains expressing myc-cyclin C (pLR337) were grown to midlog phase (0 hr) and then treated with increasing amounts of H2O2 for 1 hour. Cyclin C levels were determined by Western blot analysis of immunoprecipitates. Tub1 levels were used as a loading control. (d) Quantification of cyclin C levels in (c).
