The Cell Wall Sensors Mtl1, Wsc1, and Mid2 Are Required for Stress-Induced Nuclear to Cytoplasmic Translocation of Cyclin C and Programmed Cell Death in Yeast

Table 2

Primers used in this study.

Name	Mutation created	Oligonucleotide
MID2 F1	mid2Δ	5′ TCGTTGAAGATTGGACATATAAAATACGCAAATCATAGTCGGATCCCCGGGTTAATTAA 3′
MID2 R1	mid2Δ	5′ AGGAATGAAAAGTAGCCATAAGCACTAAATGATATCAATGAATTCGAGCTCGTTTAAAC 3′
WSC1 F	wsc1Δ	5′ GTA AAC TCG ACC AGG CAC 3′
WSC1 R	wsc1Δ	5′ TATATCGTCTTTCAACGCGG 3′
MTL1 F1	mtl1Δ	5′ TTAACTTACTCCCAGTTAGTATAATATAAGTAGTTAAGGTCGGATCCCCGGGTTAATTAA 3′
MTL1 R1	mtl1Δ	5′ TTAAGAAGAAAAGTTATGGCAAAGCTGCTTTCGCTATGATGAATTCGAGCTCGTTTAAAC 3′
MTL1 F2	MTL1-3HA	5′ TATTACACGAAACCAAACAACGGCTTAAATATCACGAACTATCGGATCCCCGGGTTAATTAA 3′
MTL1 F4	PGAL-MTL1	5′ TTTAAACACTTCTAGTTCATTTCGGGTTGGTTCGATCTTGGAATTCGAGCTCGTTTAAAC 3′
MTL1 R2	PGAL-MTL1	5′ TTGAAGCAGAGCTCTTCTTCCTGGTCGGATTGCAGCTTGCCATTTTGAGATCCGGGTTTT 3′
N42A-F	Mtl1^N42A	5′ GCAGGAGTTGGTTCCAGCTGCTAGCACAACATCGAGCAC 3′
N42A-R	Mtl1^N42A	5′ GTGCTCGATGTTGTGCTAGCAGCTGGAACCAACTCCTGC 3′
N547A-F	Mtl1^N547A	5′ GAAACCAAACAACGGCTTAGCTATCACGAACTATTAAATC 3′
N547A-R	Mtl1^N547A	5′ GATTTAATAGTTCGTGATAGCTAAGCCGTTGTTTGGTTTC 3′
KCO1234	Amplify gfa1-1	5′ CGCTTACAAGAAAGCATTC 3′
KCO1235	Amplify gfa1-1	5′ GTCTAATTTAGGGCTGCAAC 3′

Oligonucleotides used in this study with their accompanying mutation identified.