Mitochondrial-Targeted Two-Photon Fluorescent Probes for Zinc Ions, , and Thiols in Living Tissues
Figure 2
Images of a rat hippocampal slice stained with 20 μM SZn2-Mito for 1 h. (a) Bright-field images of the CA1-CA3 regions as well as dentate gyrus (DG) at 10x magnification. (b) 10 TPM images along the z-direction at the depths of approximately 100–200 μm were accumulated. (c–e) Magnification at 40x in the DG regions (red circle in (b)) at a depth of ~100 μm (c) before and (d) after addition of 150 μM DTDP to the imaging solution. (e) After addition of 10 μM CCCP to (d). Scale bars: (a) 300 and (e) 75 μm [10].
