Mitochondrial-Targeted Two-Photon Fluorescent Probes for Zinc Ions, , and Thiols in Living Tissues
Figure 3
(a–d) Pseudocolored ratiometric TPM images of Raw 264.7 cells incubated with 3 μM (a) SHP-Mito and (d) 1-SHP. (b and c) Cells were pretreated with (b) PMA (1 μg/mL) for 30 min and (c) 200 μM H2O2 for 30 min. (e) Average intensity ratios in figures (a–d). Images were acquired using 750 nm excitation and fluorescent emission windows: blue = 400–470 nm and yellow = 530–600 nm. Scale bar = 15 μm. Cells shown are representative images from replicate experiments () [11].
